dCMP, 2′-Deoxycytidine 5′-monophosphate, acidum liberum CAS: 1032-65-1
| Catalogue Number | XD90549 |
| Product Name | dCMP, 2'-Deoxycytidine 5'-monophosphate liberae acidi |
| CAS | 1032-65-1 |
| Formulae hypotheticae | C9H14N3O7P |
| M. Pondus | 307.20 |
| Repono Details | -15 ad -20 °C |
| Tariff Code harmonized | 29349990 |
Product Specification
| Aspectus | Firmus |
| Assay | 99% |
Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidini (PSI-6130) potens est inhibitor specifici viri hepatitis C (HCV) RNA synthesis in cellulis Huh-7 replicon.Ad polymerasium HCV NS5B RNA inhibendum, PSI-6130 phosphorylatae formae 5'-triphosphaerae debent esse.Phosphorylatio PSI-6130 et inhibitionis HCV NS5B investigata est.phosphorylatio PSI-6130 ab humano 2'-deoxycytidine kinasi (dCK) et uridine-cytidine kinasi 1 (UCK-1) mensurata est utendo reactionem spectrophotometricam copulatam.PSI-6130 subiecta esse monstratum est pro dCK purgato, cum Km 81 microM et kcat 0.007 s-1, sed pro UCK-1.PSI-6130 monophosphata (PSI-6130-MP) efficaciter phosphoryllata erat ad diphosphatam et postea ad triphosphatum humanum recombinante UMP-CMP kinase et diphosphate nucleoside kinase, respectively.Inhibitio generis silvestrium et mutatae (S282T) HCV NS5B RNA polymerases studuerunt.Status stabilis inhibitionis constantis (Ki) pro PSI-61 30 triphosphate (PSI-6130-TP) cum enzyme silvestri typus erat 4.3 microM.Similes eventus consecuti sunt cum triphosphate 2'-C-methyladenosino (Ki=1.5 microM) et 2'-C-methylcytidine triphosphato (Ki=1.6 microM).NS5B cum mutatione S282T, quae repugnare notum est ad 2'-C-methyladenosinum, inhibitum fuit ab PSI-6130-TP tam efficaciter quam generis silvestris.Incorporatio PSI-6130-MP in RNA catalyzata purificata NS5B RNA polymerasis consecuta est in catena terminationis.
