Biosynthesis proteoglycanorum et glycosaminoglycanorum coram p-nitrophenyl-xyloside studuit utens primaria rat ovarii granulosa cellula culturae systematis.Additio xyloside p-nitrophenyl-xyloside in medium culturae cellae causatur circa 700% incrementa incorporationis [35S] sulfatae (ED50 ad 0.03 mM) in macromoleculas, quae liberum chondroitin sulfate catenis in xyloside et proteoglycans nativo initiatum incluserunt.Liberum chondroitin sulfatis catenis in xyloside initiatis prope solum in medium secretae erant.Magnitudo hypothetica chondroitin sulfatis catenis ab 40.000 usque ad 21000 decrevit, ut incorporatio tota [35S] sulfate aucta est, suggerens auctam synthesim chondroitin sulfatis perturbasse normalem mechanismum catenae glycosaminoglycanae terminationis.Biosynthesis sulfatis heparae proteoglycanorum ab circiter 50% redacta est, verisimile ob competitions in ambitu praecursorum UDP-sugarorum.[35S] Incorporatio sulfate inclusa est addita cycloheximide cum dimidio temporis initiali circa 2 hr coram xyloside, quod in absentia xyloside erat 20 min.Differentia verisimile refert turnover rate glycosaminoglycanae capacitatis totius summae.Turnover rate of glycosaminoglycans synthesisandi capacitatis ovarii in cellulis granulosis servata multo brevior fuit quam quae in chondrocytis observata est, cum relativum dominatum proteoglycanum biosyntheticae activitatis metabolicae in tota cellularum metabolicae reflectens.
