A beta-glucosidase gene (bgl3) ex Streptomyces sp.QM-B814 (American Type Culture Collection 11238) perplexum est per complementum functionis betae glucosidasi-negativae mutant Streptomyces lividans.Tabula aperta lectionis 1440 nucleotidum descriptam a polypeptide ab acidis 479 amino sequendo inventa est.Dapibus encoded (Bgl3) maximam similitudinem (per 45% identitatis) cum beta-glycosidase e glycosyl hydrolasi familia-1 ostendit.Cloned enzyme, sequens ammonium sulphate praecipitatio purgatum et duos gradus chromatographicos, est monomeric cum massa hypothetica 52.6 kDa, secundum massa spectrometriae determinata, et punctum isoelectricae pI 4.4.Enzyme videtur esse beta-glucosidase cum specificitate lata subiecta, activa in cellooligomers, et motus transglycosylationem facit.Extimationis valores Vm apparentes pro p-nitrophenyl-beta-D-glucopyranoside et cellobiosi sunt 0.27 mM et 7.9 mM, respective.Valores Ki pro glucose et delta-gluconolactone, p-nitrophenyl-beta-D-glucopyranoside substrati, sunt 65 mM et 0.08 mM, respective.Enzyme purgatus optimum pH habet pH 6,5 et optimum temperatura actionis est 50 gradus
